ASSOCIATION BETWEEN VEGF +936C>T GENE POLYMORPHISM WITH GASTRIC PREMALIGNANT LESIONS AND VEGF LEVELS IN HELICOBACTER PYLORI PATIENTS

Gontar Alamsyah Siregar, Zaimah Z Tala, Ginanda Putra Siregar

Abstract


The objective of this study was to evaluate the association between VEGF +936 C>T gene polymorphism with
gastric premalignant lesions as well as the serum VEGF levels. This cross-sectional study included 80 H. pylori
patients at Haji Adam Malik General Hospital and Permata Bunda General Hospital, Medan, Indonesia. Diagnosis of H. pylori infection was
made if positive results of 􀄦⁴C-UBT and/orCLO test were found. If any 􀃶ndings such as chronic atrophic gastritis, intestinal metaplasia, or
dysplasia were present in microscopic evaluation, a diagnosis of gastric premalignant lesion was made. Real time polymerase chain reaction
(RT-PCR) was used to examine VEGF +936C>T gene polymorphism. Level of circulating VEGF was determined using serum samples. Data
were collected and analyzed using SPSS version 22. There was no signi􀃶cant association between VEGF +936 C>T polymorphism and gastric
premalignant lesions (p>0.05). Difference in serum VEGF levels between genotypes and alleles of VEGF +936 C>T polymorphism were not
signi􀃶cant (p=0.179 and p=0.076 respectively). VEGF +936 C>T polymorphism was not associated with an increased risk of gastric
premalignant transformation and serum VEGF levels in H.pylori patients.


Keywords


Gastric Premalignant Lesion, Helicobacter Pylori, Vegf, Polymorphism

Full Text:

PDF

References


Sepulveda AR, Patil M. Practical Approach to the Pathologic Diagnosis of Gastritis. Arch Pathol Lab Med. 2008;132(10):1586-93.

Fox JG, Wang TC. Inflammation, atrophy and gastric cancer. The Journal of Clinical Investigation. 2007;17:60-9.

Inoue M. Changing epidemiology of Helicobacter pylori in Japan. Gastric Cancer. 2017;20(Suppl 1):3-7.

Du Y, Bai Y, Xie P, et al. Chronic gastritis in China: A national multi-center survey. BMC Gastroenterology. 2014;14:21-30.

Haziri A, Shkololli AJ, Gashi Z, Berisha D, Haziri A. Helicobacter pylori infection and precancerous lesions of the stomach. Medicinski Arhiv. 2010;64:248-9.

Tahara T, Arisawa T, Shibata T, Nakamura M, Yamashita H, Yoshioka D, et al. Effect of Polymorphisms in the 3’-Untranslated Region (3’-UTR) of VEGF Gene on Gastric Premalignant Condition. Anticancer research. 2009; 29:458-90.

Guan X, Zhao H, Niu J, Tan D, Ajani JA, Wei Q. Polymorphisms of TGFβ1 and VEGF genes and survival of patients with gastric cancer. J Exp Clin Cancer Res. 2009a;28:94.

Jemal A, Siegel R, Ward E, et al. Cancer statistics, 2008. CA Cancer J Clin. 2008;58(2):71-96.

Chekhonin VP, Shein SA, Korchagina AA, Gurina OI. VEGF in tumor progression and targeted therapy. Current Cancer Drug Targets. 2013;13(4):423-43.

Chung HW, Lim J-B. Role of the tumor microenvironment in the pathogenesis of gastric carcinoma. World J Gastroenterol: WJG. 2014;20(7):1667-80.

Bae SJ, Ahn DH, Hong SP, Kang H, Hwang SG, Oh D, et al. Gender-specific association between polymorphism of vascular endothelial growth factor (VEGF 936 C>T) gene and patients with stomach cancer. Yonsei Med J. 2008;49(5):783-91.

Ghanaei FM, Sanaei O, Joukar F. Clinical Validation of an Office-Based 14C-UBT (Heliprobe) for H. pylori Diagnosis in Iranian Dyspeptic Patients. Gastroenterol Res Pract. 2011 (2011): Article ID 930941

Rojborwonwitaya J, Vijitjunykul N. Comparison of the Accuracy of Two Commercial Rapid Urase Tests, CLOtest®, and Pronto Dry®, in detecting Helicobacter pylori Infection. Thai J Gastroenterol. 2005:6(2):55-60.

Zhou LP, Luan H, Dong XH, Jin GJ, Man DL, Shang H. Vascular endothelial growth factor +936C/T polymorphism and gastric cancer risk: A meta-analysis. Experimental and Therapeutic Medicine 2011(2): 931 – 936

Kim JG, Sohn SK, Chae YS, Cho YY, Bae HI, Yan G, et al. Vascular endothelial growth factor gene polymorphisms associated with prognosis for patients with gastric cancer. Ann Oncol. 2007;18:1030-6.

Tzanakis N, et al. Vascular Endothelial Growth Factor Polymorphisms in Gastric Cancer Development, Prognosis, and Survival. Journal of Surgical Oncology 2006(94): 624 – 630

Goldberg-Cohen I, Furneauxb H, Levy AP. A 40-bp RNA Element That Mediates Stabilization of Vascular Endothelial Growth Factor mRNA by Hur 2002; 277(16):13635 – 13640

Ke Q, Liang J, Wang LN. Potentially functional polymorphisms of the vascular endothelial growth factor gene and risk of gastric cancer. Mol Carcinog. 2008;47(8):647-51.

Guan X, Zhao H, Niu J, Tang D, Ajani JA, Wei Q. The VEGF -634G>C promoter polymorphism is associated with risk of gastric cancer. BMC Gastroenterology 2009; 9:77

Liu W, Dong Z, Hu R, Wang C. Association of Vascular Endothelial Growth Factor (VEGF) Gene Polymorphisms With Gastric Cancer and Its Development, Prognosis, and Survival. Technology In Cancer Research & Treatment 17: 1 – 8

Corvalan AH, Carrasco G, Saavedra K (2012) The genetic and epigenetic bases of gastritis. In Mozsik G (ed) Current Topics in Gastritis, InTech, London, pp.79-95).

Renner W, Kotschan S, Hoffmann C, et al. A common 936 C/T mutation in the gene for vascular endothelial growth factor is associated with vascular endothelial growth factor plasma levels. J Vasc Res. 2000;37:443–8.


Refbacks

  • There are currently no refbacks.