EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF HYDROETHANOLIC EXTRACT OF WHOLE PLANT OF CLEMATIS BUCHANANIANA DC AGAINST PARACETAMOL INDUCED LIVER INJURY IN WISTAR ALBINO RATS.

Anita Singh, Manoj Bisht

Abstract


Objective: To investigate the hepatoprotective activity of hydroethanolic extract of whole plant of Clematis
buchananiana against Paracetamol-induced liver injury in wistar albino rats. Belong to family Ranuculaceae. The
degree of protection was measured by estimation of serum biochemical parameters, histopathology study.
Method: The albino wistar rats (120-180gm) were divided into 6 group 5 animals in each, Group I: Received distilled water (5ml/kg. p.o) once
daily, and served as normal control. Group II: Received paracetamol suspension (640 mg/kg suspended in 1% methyl cellulose; orally as toxin
control. Group III: Received standard drug Silymarin (25 mg/kg. p.o.) + paracetamol suspension (640 mg/kg suspended in 1% methyl cellulose;
orally once daily Group IV, V, VI administered HEECB at different doses300, 400, 500 mg/kg orally + paracetamol suspension (640 mg/kg
suspended in 1% methyl cellulose; for 21 days. And collect blood from experimental animals by retrorbital puncture for estimation of
biochemical parameters and other parameter also evaluate like physical histological changes in livers of rats.
Results: Experimental nding reveal that Paracetamol produce signicant change in physical (increase liver weight) biochemical (increase
alkaline phosphate, serum glutamic oxalacetic transaminase, serum glutamic pyuruvic transaminase, total protein, total bilirubin, direct
bilirubin and decrease the level of total protein and albumin) histological (damage to hepatocyte) and in liver parameters. Pretreatment with
extract signicantly minimization of physical, biochemical, histological and functional change induced by Paracetamol in liver.
Conclusion: Experimental data and analysis of different parameter declare that hydroethanolic extract of Clematis buchananiana could be a
useful hepatoprotective agent and it has signicant hepatoprotection potential it is possible due to their active constituent alkaloids. However
further study still needed to be causes on exposure of extract to human beings.


Keywords


Clematis Buchananiana, Paracetamol, Hepatoprotective, Alkaline Phosphate, Serum Glutamic Oxalacetic Transaminase, Serum Glutamic Pyuruvic Transaminase.

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