Anita Singh, Manoj Bisht


Objective: To investigate the hepatoprotective activity of hydroethanolic extract of whole plant of Clematis
buchananiana against Paracetamol-induced liver injury in wistar albino rats. Belong to family Ranuculaceae. The
degree of protection was measured by estimation of serum biochemical parameters, histopathology study.
Method: The albino wistar rats (120-180gm) were divided into 6 group 5 animals in each, Group I: Received distilled water (5ml/kg. p.o) once
daily, and served as normal control. Group II: Received paracetamol suspension (640 mg/kg suspended in 1% methyl cellulose; orally as toxin
control. Group III: Received standard drug Silymarin (25 mg/kg. p.o.) + paracetamol suspension (640 mg/kg suspended in 1% methyl cellulose;
orally once daily Group IV, V, VI administered HEECB at different doses300, 400, 500 mg/kg orally + paracetamol suspension (640 mg/kg
suspended in 1% methyl cellulose; for 21 days. And collect blood from experimental animals by retrorbital puncture for estimation of
biochemical parameters and other parameter also evaluate like physical histological changes in livers of rats.
Results: Experimental nding reveal that Paracetamol produce signicant change in physical (increase liver weight) biochemical (increase
alkaline phosphate, serum glutamic oxalacetic transaminase, serum glutamic pyuruvic transaminase, total protein, total bilirubin, direct
bilirubin and decrease the level of total protein and albumin) histological (damage to hepatocyte) and in liver parameters. Pretreatment with
extract signicantly minimization of physical, biochemical, histological and functional change induced by Paracetamol in liver.
Conclusion: Experimental data and analysis of different parameter declare that hydroethanolic extract of Clematis buchananiana could be a
useful hepatoprotective agent and it has signicant hepatoprotection potential it is possible due to their active constituent alkaloids. However
further study still needed to be causes on exposure of extract to human beings.


Clematis Buchananiana, Paracetamol, Hepatoprotective, Alkaline Phosphate, Serum Glutamic Oxalacetic Transaminase, Serum Glutamic Pyuruvic Transaminase.

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Mishra, G., Khosa, R. L., Singh, P., &Jha, K. K. (2015). Hepatoprotective potential of ethanolic extract of Pandanusodoratissimus root against paracetamol-induced hepatotoxicity in rats. Journal of pharmacy &bioallied sciences, 7(1), 45.

Graham, G. G., & Scott, K. F. (2005). Mechanism of action of paracetamol. American journal of therapeutics, 12(1), 46-55.

PREScon, L. F. (1983). Paracetamoloverdosage. Pharmacological considerations and clinical managemente. Drugs, 25, 290-314.

Mitra, S. K., Venkataranganna, M. V., Sundaram, R., &Gopumadhavan, S. (1998). Protective effect of HD-03, a herbal formulation, against various hepatotoxic agents in rats. Journal of Ethnopharmacology, 63(3), 181-186.

Nadkarni, A. K., &Nadkarni, A. K. (1976). In Indian MateriaMedica, Popular Prakashan (Pvt) Ltd. Bombay, 1, 1199.

Singh, G., &Rawat, G. S. (2011). Ethnomedicinal survey of Kedarnath wildlife sanctuary in western Himalaya, India. Indian Journal of Fundamental and Applied Life Sciences, 1(1), 35-46.

Manoj, B., Mahak, A., Varsha, T., Manoj, B., Abhishek, T., 2017. Evaluation of In-VitroAntiangiogenesis activity on methanolic extract of Clematis BuchanianaDC Asian J Pharm Clin Res. 10, 227-229

TSUKAMOTO, H., & LU, S. C. (2001). Current concepts in the pathogenesis of alcoholic liver injury. The FASEB Journal, 15(8), 1335-1349.

Mitchell, J. R., Jollow, D. J., Potter, W. Z., Davis, D. C., Gillette, J. R., & Brodie, B. B. (1973). Acetaminophen-induced hepatic necrosis. I. Role of drug metabolism. Journal of Pharmacology and Experimental Therapeutics, 187(1), 185-194.

McGill, M. R., &Jaeschke, H. (2013). Metabolism and disposition of acetaminophen: recent advances in relation to hepatotoxicity and diagnosis. Pharmaceutical research, 30(9), 2174-2187.

Luna, Kuiken, T., Wobeser, G., Leighton, F. A., Haines, D. M., Chelack, B., Bogdan, J.& Riva, J. (1999). Pathology of Newcastle disease in double-crested cormorants from Saskatchewan, with comparison of diagnostic methods. Journal of wildlife diseases, 35(1), 8-23.

Rao, P. G., Rao, G., Ramnarayan, K., & Srinivasan, K. K. (1992). Effect of hepatogard on paracetamol induced liver injury in male albino rats. Indian drugs, 30(1), 40-47.

Organisation for Economic Co‐operation and Development (OECD). (1997). OECD Guidelines for the Testing of Chemicals. Section 4: Health Effects.

Kshirsagar, A. D., Mohite, R., Aggrawal, A. S., &Suralkar, U. R. (2011). Hepatoprotective medicinal plants of Ayurveda-A review. Asian Journal of Pharmaceutical and clinical research, 4(3), 1-8.

Khare, C. P. (2008). Indian medicinal plants: an illustrated dictionary. Springer Science & Business Media.

Nordmann, R., Ribière, C., &Rouach, H. (1992). Implication of free radical mechanisms in ethanol-induced cellular injury. Free Radical Biology and Medicine, 12(3), 219-240.


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