G. Velvizhi, Sucilathangam G, Srinivasananth K, Revathy C


BACKGROUND AND OBJECTIVES: Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of
Hospital-acquired infections in many countries. Mainly Health Care workers (HCWs) are at risk for Methicillin-
Resistant Staphylococcus aureus (MRSA) carriage.
MATERIALS AND METHODS: A Cross-sectional study was conducted among 50 Health Care Workers (HCWs) screened for carriage of
MRSA. Swabs of Anterior nares were taken, transported and inoculated on blood agar and incubated at 37°C for 24 hours. After incubation,
growth was identied as Staphylococcus aureus on the basis of colony morphology, Gram stain, and catalase and coagulase test. (Slide and
Tube)MRSA was identied using Cefoxitin disc diffusion methods.
RESULTS: Overall carriage rate was 4% with 2% in theatre staffs and nursing staffs. All the MRSA isolates were resistant to Penicillin sensitive
to all the tested antibiotics. Health Care workers were the potential colonizers of Methicillin Resistant Staphylococcus aureus.
CONCLUSIONS: These carriers may serve as reservoir and disseminator of MRSA, and should be treated with Mupirocin 3 times daily for 5
days prolonged hospital stay and improper hand washing were found to be the major risk factors for MRSA colonization, although it would
require screening of larger numbers before arriving at any denite conclusions. There is need for MRSA control policies in hospitals.


Methicillinresistant Staphylococcusaureus(MRSA),HealthCare workers,Carriage,Anterior nares,Cefoxitin Disc Diffusion test

Full Text:



.Aires-de-sousa M, Conceicao T, de Lencastre H:Unusually high prevalence of nosocomialpantonvalentineleckociden-positive Staphylococcus aureusisolates in Cape Verde islands. JClinMicrobiol 2006;44(10):3790–3793.

.Ahmed MO, Elramalli AK, Amri SG, AbuzwedaAR,Abouzeed YM: Isolation and screeningof Methicillin Resistant Staphylococcus aureus from health care workers in Libyan hospitals.EastMediterr Health J2012; 18(1):37–42.

.Omuse G, Kariuki S, Revathi G: Unexpected absence of Methicillin Resistant Staphylococcusaureus nasal carriage by healthcare workers in a tertiary hospital in Kenya. J Hosp Infect 2012;80(1):71–73.

.Truong H, Shah SS, Ludmir J, Twananana EO, BafanaM, Wood SM, MoffatH, SteenhoffAP:Staphylococcusaureusskin and soft tissue infections at a tertiary hospital in Botswana. S AfrMed J 2011; 101(6):413–416.

.Eveillard M, Martin Y, Hidri N, BoussougantY, Joly-GouillouML.Carriage of MethicillinResistant Staphylococcus aureusamong hospital employees:prevalence, duration, and transmission to households. Infect Control HospEpidemiol.2004; 25: 114-120.

.Haas JP and Larson EL. Academic Emergency Medicine 2008; 15(4): p.393-396.

.Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial DiskSusceptibility Tests; Approved Standard-Eleventh Edition and PerformanceStandards forAntimicrobial Susceptibility Testing: Twenty Third Informational Supplement. CLSIDocumentM02-A11 and M100-S23.Wayne, PA: Clinical and Laboratory Standard Institute;2013.

.Reboli AC, John JF, Platt CG, CanteyJR.Methicillin-resistant Staphylococcus aureusoutbreak at a veterans affairs medical centre: importance of carriage of the organism by hospital personnel. Infect Control Hosp Epidemiol1990 ;11: 291-296.

.Angelo P, Lorenzotti S, Ferrari L , Granata L, Signorini L, Carnevale G. Low rates of nasal colonization with Methicillin-Resistant Staphylococcus aureus among staff members of an Italian hospital. Infect Control HospEpidemiol2006; 27: 218-220.

.Jimei D, Chun C, Baixing D, Jinjing T, Zhiqiang Q, Chris al.Molecular Characterization and Antimicrobial Susceptibility of Nasal Staphylococcus aureus Isolates from a Chinese Medical College Campus.2011;11(6):1-5.

. Baragundi MC, Kulkarni RB, Sataraddi J. Prevalence of Inducible and Constitutive Clindamycin Resistance among the Nasal Isolates of Staphylococci; Journal of Clinical and Diagnostic Research. 2013;7(8):1620-1622.

.Yazgi H, Ertek M, Ozbek A, Kadanali A. Nasal carriage of Staphylococcus aureus in hospital personnel and the normal population and antibiotic resistance of the isolates. MikrobiyolBul 2003;37:137‑42.

.Al‑Abdli NE, Baiu S. Nasal carriage of Staphylococcus in health care workers in Benghazi hospitals. Am J Microbiol Res 2014;2:110‑2.

.Jadhavar SR, Modak MS, Junaid H.Screening for Methicillin Resistant Staphylococcus Aureus among Healthcare Workers in a Tertiary Care Hospital;International Journal of Health Sciences & Research.2013;5(3):1-115.

Nagarajan A, Saravavan M, Padma K. Indian J Microbiol 2013;53(3):288-290.

Chinmoy S, Jayashree K, Sayantan B, Suranjan P, Inam DK. Nasal Carriage Of Methicillin Resistant Staphylococcus Aureus Among HealthCare Workers In A Tertiary Care Hospital 2015; 20(1) :66-67.


  • There are currently no refbacks.