Prevalence and Antimicrobial Susceptibility of Staphylococcus aureus strain from Clinical Isolates at Gwalior Medical College Hospital

Saroj Kothari, Anubhuti Khare, Vaibhav Misra

Abstract


Present study was undertaken to find the prevalence & antimicrobial sensitivity of Staphylococcus aureus in clinical
isolates in tertiary care hospital. Various samples were collected for isolation & antimicrobial susceptibility testing of
S.aureus. Out of 5000 samples collected 1684 showed growth in culture media. A total of 255 Staphylococcus were isolated & subjected to
antimicrobial susceptibility. High antimicrobial resistance was seen with common antimicrobial agents such as erythromycin, ampicillin,
cefixime & ceftazidime+clavulinic acid as 78%, 71%, 71% & 63% respectively. Mean highest sensitivity to linezolid (84%), vancomycin (83%) &
doxycycline (82%), whereas mean intermediate antibiotic sensitivity to amikacin (77%), levofloxacin (72%), amoxiclav (64%) & piperacillin
(56%) were observed. Present study showed that Staphylococcus aureus (15%) were resistant to commonly used antibiotics & did not show
100% sensitivity to linezolid & vancomycin. us, there is urgent need to regulate use of antibiotics in hospital for preventing the spread of
antimicrobial resistance.


Keywords


Staphylococcus aureus, antimicrobial resistance, hospital infection, clinical isolates.

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References


REFRENCES

BisenP S, DebnathM, PrasadG B, editors (2012). Microbes: Concepts and Applications (1sted.) (P 3-4). New Jersey: John Wiley & Sons.

Rajaduraipandi K, Mani K R, Panneerselvam K, Mani M, Bhaskar M, Manikandan P (2006). Prevalence and antimicrobial susceptibility pattern of methicillin resistant Staphylococcus aureus: A multicentre study. Indian J Med Microbiol.,24,34-8.

Qureshi AH, Rafi S, Qureshi SM, Ali AM (2004). The current susceptibility patterns of methicillin resistant Staphylococcus aureus to conventional anti Staphylococcus antimicrobials at Rawalpindi. Pak J Med Sci., 20, 361–4.

Clinical and Laboratory Standards Institute (2014). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement (M100-S24); Table 3A; Vol. 34(1): 110-3

Ginawi I, Saleem M, Sigh M, Vaish AK, Ahmad I, Srivastava VK, et al (2014).Hospital Acquired Infections Among Patients Admitted in the Medical and Surgical Wards of a Non-Teaching Secondary Care Hospital in Northern India. J Clin Diagn Res., Feb 8(2), 81–83.

Yasmeen F , Sarwar MI, Hakeem A, Sherwani SK , Hussain MS , Zeb M, et al (2014). Identification of Staphylococcus aureus in Pus samples and its Anti-microbial Susceptibility against Imipenem,Tobramycin and Linezolid. IJBMSP, 4(1), 9-12.

Asati RK (2013). Antimicrobial Sensitivity Pattern of Staphylococcus aureus isolated from Pus Fromtertiary Care Hospital, Surendranagar, Gujarat and Issues Related to the Rational Selection of Antimicrobials. Sch. J. App. Med. Sci., 1(5), 600-605.

Baron S, editor (1996). Medical Microbiology (4th edition). Galveston (TX): University of Texas Medical Branch at Galveston. Available from: https://www.ncbi.nlm.nih.gov/books/NBK7627/

Laurence L. B, editor., Bruce A. C, Bjorn C. K, assoc. editors (2011). Goodman & Gilman’s The Pharmacological Basis of Therapeutics(12thed.) (P.1486, 1537, 1541, 1508, 1472, 1530). China: Mc Graw Hill.

Rodvold KA, McConeghy KW (2014). Methicillin-Resistant Staphylococcus aureus Therapy: Past, Present, and Future. Clinical Infectious Diseases, 58(S1), S20–7.

Jevons MP (1961). ‘Celbenin’-resistant Staphylococci. BMJ,1, 124.

Patel AK, Patel KK, Patel KR, Shah S, Dileep P (2010). Time trends in the epidemiology of microbial infections at a tertiary care center in west India over last 5 years. J Assoc Physicians India,58 (Suppl), 37-40.

Gopalakrishnan R, Sureshkumar D (2010). Changing trends in antimicrobial susceptibility and hospital acquired infections over an 8 year period in a tertiary care hospital in relation to introduction of an infection control programme. J Assoc Physicians India, 58 (Suppl), 25-31.

D’Souza N, Rodrigues C, Mehta A (2010). Molecular characterization of Methicillin-resistant Staphylococcus aureus with emergence of epidemic clones of sequence type (ST) 22 and ST 772 in Mumbai. India. J Clin Microbiol.,48, 1806-11.

Saikia L, Nath R, Choudhury B, Sarkar M (2009). Prevalence and antimicrobial susceptibility pattern of methicillin-resistant Staphylococcus aureus in Assam. Indian Journal of Critical Care Medicine : Peer-reviewed, Official Publication of Indian Society of Critical Care Medicine, 13(3), 156-158.

Pai V, Rao VI, Rao SP (2010). Prevalence and Antimicrobial Susceptibility Pattern of Methicillin-resistant Staphylococcus Aureus [MRSA] Isolates at a Tertiary Care Hospital in Mangalore, South India. Journal of Laboratory Physicians, 2(2), 82-84.

Joshi S, Ray P, Manchanda V, Bajaj J, Chitnis D S, Gautam V, et al (2013). Methicillin resistant Staphylococcus aureus (MRSA) in India: Prevalence & susceptibility pattern. Indian Network for Surveillance of Antimicrobial Resistance (INSAR) group, India. Indian J Med Res., 137, 363-369.

Mandelia C, Shenoy S, Garg Y (2012).Antibiotic sensitivity pattern of community associated-methicillin resistant Staphylococcus aureus. Rev. Soc. Bras. Med. Trop., 45(3).


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