Neha Yadav, Anjana Pandey


Black gram species are fast- growing legume which usually grows in South-Asian region. The seed sample when boiled with an acidic iron solution of known iron concentration form a colored solution. The iron present bound to 2, 2`-bipyridine is inversely related to the amount of phytic acid present which was detected through spectrophotometer. The phytic acid and inorganic phosphate concentration in 34 samples which includes 31 black gram samples, 1 pigeon pea, 1 red lentil and 1 split bengal gram was determined. The phytic acid concentration range from 2.847 to 23.6 mg/g sample whereas inorganic phosphorous concentration range from 56.4 to 121.55 µg/g sample. Considerable variations were observed for both PA and IP content in black gram samples. Consumption of black gram grains with low phytic acid (lpa) will increase the absorption of nutrients in humans.


Black gram, Phytic acid, Inorganic phosphorus, Seeds

Full Text:



Vinod Janardan Dhole, Kandali Srinivasalu Reddy( 2 0 1 5 ) Genetic variation for phytic acid content in black gram (Vigna radiata L. Wilczek). T h e Crop Journal- 3, 157 – 162

Kenan Dost, Ozge Tokul(2006) Determination of phytic acid in wheat and wheat products by reverse phase high performance liquid chromatography. Analytica Chimica Acta 558 22–27

Máté Hídvégi and Radomir Lásztity (2002) Phytic Acid Content of Cereals and Legumes and Interaction with Proteins. Periodica Polytechnica Ser. Chem. Eng. Vol. 46, No. 1–2, pp. 59–64

Andréia Jacinto Agostinho & Woodland de Souza Oliveira & Daniela Santos Anunciação & Josué Carinhanha Caldas Santos(2010) Simple and Sensitive Spectrophotometric Method for Phytic Acid DeterminationinGrains. Food Anal. Methods

Josiane Meire Tolotti Carneiro, Elias Ayres Guidetti Zagatto, João Luis Machado Santos, José Luis Fontes Costa Lima(2002). Spectrophotometric determination of phytic acid in plant extracts using a multi-pumping flow system. Analytica Chimica Acta 474 161–166

Pave1 Blatny," Frantisek Kvasnicka, and Ernst Kenndler(1995) Determination of Phytic Acid in Cereal Grains, Legumes, and Feeds by Capillary Isotachophoresis J. Agric. Food Chem., 43, 129-133 129

Wolfgang Hauga and Hans-Joachim Lantzsch(1983). Sensitive Method for the Rapid Determination of Phytate in Cereals and Cereal Products. J. Sci. Food Agric., 34, 1423-1426

Muraoka, S., & Miura, T. (2004). Inhibition of xanthine oxidase by phytic acid and its antioxidative action. Life sciences, 74(13), 1691-1700.

Dolan, L. C., Matulka, R. A., & Burdock, G. A. (2010). Naturally occurring food toxins. Toxins, 2(9), 2289-2332.

Jagadeesh, S., & Banerjee, P. P. (2006). Inositol hexaphosphate represses telomerase activity and translocates TERT from the nucleus in mouse and human prostate cancer cells via the deactivation of Akt and PKCα. Biochemical and biophysical research communications, 349(4), 1361-1367.

Kapral, M. A. Ł. G. O. R. Z. A. T. A., Wawszczyk, J., Hollek, A., Dymitruk, D., & Weglarz, L. (2012). Inhibitory effect of inositol hexaphosphate on metalloproteinases transcription in colon cancer cells stimulated with phorbol-12-myristate 13-acetate. Acta Pol Pharm, 69(6), 1307-12.

Thompson, L. U., Button, C. L., & Jenkins, D. J. (1987). Phytic acid and calcium affect the in vitro rate of navy bean starch digestion and blood glucose response in humans. The American journal of clinical nutrition, 46(3), 467-473.


  • There are currently no refbacks.