Dr Rahul Agarwal, Dr Naveen Saxena


BACKGROUND- Japanese encephalitis is a mosquito borne viral encephalitis caused by Japanese encephalitis virus. Japanese encephalitis (JE)
virus was first isolated from Japan in 1935. Than it spread worldwide and becoming a major public health problem due to its high epidemic potential
and high case fatality rate. In our study, suspected cases of JE were belong to Keshoraipatan village in Bundi district, Rajasthan which is unusual in
that area and the state of Rajasthan.
MATERIAL AND METHODS- Serum sample was collected properly from each patient with clinical history of high grade fever, headache,
vomiting and altered sensorium. Than these serum samples were tested for qualitative detection of Dengue IgM antibodies by NIV (National
Institute of Virology) Dengue IgM Capture ELISA kit. Out of all samples those found negative for Dengue IgM antibodies, a total of 24 samples
which had clinical suspicion of JE, were sent to the NIV (National Institute of Virology ) lab, Pune for detection of JE IgM antibodies. In NIV lab,
the serological identification of JE IgM antibodies was done by JE virus MAC ELISA test.
RESULTS- Out of 24 samples, 8 samples were found positive for JE IgM antibodies. The mean age for positive cases was 39 years. Out of 8
positive cases, 6 were females and 2 were males. Majority of the patients were belong to low socioeconomic group.
CONCLUSION- JE is now a serious public health problem in all over the world and India also. Detection of JE virus specific IgM antibodies in
patient's serum indicate recent infection which helps in the early treatment of the disease and prevent severe neuropsychiatric sequelae.


Japanese Encephalitis, Culex Mosquitoes, ELISA (Enzymed-Linked Immunosorbent Assay)

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