HISTOLOGICAL EFFECTS OF PESTICIDES ON INTERSTITIAL CELLS OF TESTIS IN ALBINO RATS

Dr. Seema Sharma, Dr. S. hamid, Dr. Sunanda Raina

Abstract


INTRODUCTION: Pesticides, though present in the environment in small quantities as compared to other contaminants such as industrial wastes
and fertilizers, account for public and scientific concern due to their high biological activity. Exposure of Pesticides not only reduced fertility but
also embryo/fetal loss, birth defects, childhood cancer, and other postnatal or functional deficits. Carbaryl is one of the most important insecticides
as it is widely produced and used which has prompted us to initiate this study .
MATERIAL AND METHODOLOGY: The present study was conducted on 40 male Wistar albino rats as experimental animals. The rats were
procured from the Animals House of the Department of Pharmacology, Government Medical College, Jammu. The rats were divided in four groups
as normal control group I, group II, group III and group IV. All the rats were group housed and were fed with standard pellet diet and water for two
weeks. After two weeks, the rats of group I were left as such and rats of groups II, III and IV were given 50 mg, 100 mg and 200 mg/kg body
weight/day of carbaryl drug in 0.2 ml of groundnut oil orally, 6 days/week for 60 days, respectively. After 60 days, all the rats were anaesthetized by
keeping them in an inverted glass jar containing large piece of cotton soaked in anaesthetic ether. The testis were dissected out from each rat and
were cut into smaller pieces. These pieces were immediately fixed in 10% formalin. The blocks were prepared for section cutting with a microtome
by paraffin wax embedding method. The sections of 7 μ thickness were mounted on glass slides and were stained by H&E and Masson's trichome
stain.
OBSERVATIONS: Interstitial spaces are showing the oedema & Leydig cells show degeneration.
CONCLUSION: These findings are highly conclusive of reproductive toxicity produced by an insecticide, Carbaryl. Moreover, intensity of toxic
effects both in peripheral and central parts increases with increase in dosage of the carbaryl drug.


Keywords


Albino rats; Testis; Carbaryl; Interstitial edema

Full Text:

PDF

References


Ernest, H. and Patricia, E.L. (1997). A Textbook of Modern Toxicology 2nd edition, (Edited by Hodgson E. and Levi P.E.). Toxicology Program North Carolina: Appleton and Lange, p. 15.

Leydig, F. (1850). Zuranatimie der mannlichen. Geschlechtsorgane und Analdrusen der Sangethiere. Z. Wiss. Zool., 2: 1-57.

Bouin, P. and Ancel, P. (1903). Recherches Sur les cellules interstitielles du testicule des mammiferes. Arch. Zool. Exptl. Gen., 1: 437-523.

Kamrin, M.A. (1997).Pesticides Profiles: Toxicity, Environmental Impact and Fate. Chemical Rubber Company (CRC) Press, pp. 136-137.

Mattison, D.R., Bogemil, R.J., Chapin, R., Hatch, M., Hendricks, A.,Jarrel, J., Barbera, A.L., La Schrader, S.M. and Seleval, S. (1990). Reproductive effects of pesticides. In "The Effects of Pesticides on Human Health" (Edited by Baker SR and Wilkinson CF). Published by Princeton Scientific, New Jersey, pp. 297-389.

Hileman, B. (1994). Environmental estrogens linked to reproductive abnormalities and cancer. Chem. Eng. New, 31: 19-23.

Bendvold, E., Gottlieb, C., Bygdeman, M. and Eneroth, P. (1991). Depressed semen quality in Swedish men from barren couples: a study over three decades. Arch. Androl., 26: 189-194.

Carlsen, E., Giwercman, A., Keiding, N. and Skakkebark N.E. (1992). Evidence for decreasing quality of semen during the past 50 years. Br. Med. J., 305: 609-613.

Afifi, N., Ramadan A., El-Aziz, M.A. and Saki, E. (1991). Influence of dimethoate on testicular and epidydmal organs, testosterone plasma level and their residues in rat. Dtsch. TierarztlWochenschr, 98: 419-423.

Abou Salem, M.E., El-Mashad, A.I. and Moustafa, S.A. (1997). Pathological male reproductivity and residues of dimethoate toxicity in albino rats. Alex. J. Vet. Sci., 13: 119-140.

Salem, D.A. and AbdElghaffar, S.K. (1998). Effect of nuvacron on reproductive system of male albino rats. Assiut Vet. Mecl. J., 39: 130-144.

Okamura, A., Kamijima, M., Shibata, E et al. (2005). A comprehensive evalution of the testicular toxicity of dichlorvos in Wister rats. Toxicol.,213: 129-137.

Presibella, K.M., Kita, D.H., Carneiro, C.B., Adrada, A.J.M. and Dalsenter,P.R. (2005). Reproductive evalution of two pesticides combined (delta methrine and endosulfan) in female rats. Reprod. Toxicol.,20: 95-101.

Rani, A., Sahai, A., Srivastava, A.K. and Rani, A. (2007).Carbaryl induced histopathological changes in the testis of albino rats. J. Anat. Soc. India, 56(1): 4-6.

Rybakova, M.N. (1966). Toxic effects of sevin on animals. Hyg. Sanit. (USSR), 31: 402-407.

Vashakidze, V.I. (1975). Effects of small doses of sevin (NMC) on gonad function following its repeated effect on white rats. Sb–Tr NII GisienyTrudaProfzabolevaniiGruz SSR, 14: 253-266.

Pant, N., Srivastava, S.C., Prasad, A.K., Shankar, R. and Srivastava,S.P. (1995). Effects of carbaryl on the rats male reproductive system. Vet. Hum. Toxicol.,37(5): 421-425.

Degraeve, N., MoutschenDahmen, M., Houbrechts, N. and Collizzi, A. (1976). The hazards of an insecticide: carbaryl used alone and in combination with nitrites. Bull. Soc. Sci. Iiege., 45: 46-56.

Kitagawa, K., Wakakura, M. and Ishikawa, S. (1977). Light microscopic study of endocrine organs of rats treated by carbamate pesticide. J. Toxicology Sci., 2: 53-60.


Refbacks

  • There are currently no refbacks.