Gahlot Gaurav PS, Ahuja Ankur, Badwal Sonia, Pandey Pankaj, Beenu Singh


Despite the fact that automation is indispensible in view of accuracy
and efficacy, the manual age old CBC methodology is still functional
due to economic considerations and non availability of Automated Cell
Counters (ACCs), particularly in the smaller laboratory in developing
countries. In 1940s, Wallace Coulter developed a blood cell analysis
[tool for quick screening of large number of blood samples. 1] Modern
ACCs work on light scatter or impedance Coulter principles. In 1968,
Dittrich and Goehde demonstrated fluorescence based cytometry by
coupling laser beam to flow device discovered by Fulwyer et al.[2] A 5-
part differential ACC counts leucocytes, enumerate percentage and
absolute number of IGs using a combined technology of cytochemistry,
focused flow impedance, and light absorbance. The ongoing
improvements in these instruments resulted in enumeration & evaluation
of blood cells with precision, speed, more accuracy and at reduced cost.


hematological parametersin chronic kidney diseases

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