IN VITRO EVALUATION OF ANTIOXIDANT ACTIVITY OF TENELIGLIPTIN BY FRAP AND NITRIC OXIDE ASSAY

Dr. Ramya Ravichandar, Dr. Anitha Elango, Dr. Akila L, Dr.Arul Amutha Elizabeth

Abstract


The aim of the study is to evaluate the antioxidant activity of the newer anti-diabetic drug by using FRAP and nitric oxide assay.
Methodology: FRAP assay was done by using Benzie and Strain method (1999). The antioxidant activity is measured by the reduction of
ferric complex (Fe3+ tripyridyltriazine) which is colourless to blue-colored ferrous complex (Fe2 tripyridyltriazine) at 593nm.This action is due +
to the electron donating antioxidants at low pH. FRAP values are calculated by comparing the absorbance change at 593 nm in test reaction
mixtures with that of the standard (ascorbic acid).Nitric oxide assay was done by using Kumar S et al. method (2008). In this method Sodium
nitroprusside in aqueous solution at physiological pH spontaneously generates Nitrite oxide which interacts with oxygen to produce Nitrite ions,
which can be measured at 550nm by spectrophotometer in the presence of Griess reagent and was compared with that of the standard (ascorbic
acid).Since Teneligliptin which is the novel DPP4 inhibitor plays a vital role in the management of type 2 diabetes mellitus we have evaluated the
antioxidant potential of this drug by using FRAP and nitric oxide assay.


Keywords


Teneligliptin, FRAP, Anti Oxidant Assay,nitric Oxide, Dpp4 Inhibitor, Ascorbic Acid

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References


Aparnaravi et al.Evaluation of radical scavenging property of sitagliptin,Journal of Chemical and Pharmaceutical Research, 2015, 7(10):273-275.

Jose PC, Vasanthi C, Darling CD, Stephen A. Evaluation of antioxidant property of vildagliptin. Int. J. Pharmacol. Toxicol. 2014;4(3):178-80.

Goda, Maki, and T. Kadowaki. "Teneligliptin for the treatment of type 2 diabetes." Drugs of today (Barcelona, Spain: 1998) 49.10 (2013): 615-629.

Morishita R, Nakagami H. Teneligliptin: Expectations for its pleiotropic action. Expert OpinPharmcother. 2015;16:417–26.

Sharma SK, Panneerselvam A, Singh KP, Parmar G, Gadge P, Swami OC. Teneligliptin in management of type 2 diabetes mellitus. Diabetes, metabolic syndrome and obesity: targets and therapy. 2016;9:251.

IFF Benzie, and JJ Strain. (1999). Ferric reducing/ antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol 299:15–27.

Kumar S, Kumar D, SAROHA K, Singh N, Vashishta B. Antioxidant and free radical scavenging potential of Citrulluscolocynthis (L.) Schrad. methanolic fruit extract. ActaPharmaceutica. 2008 Jun 1;58(2):215-20.


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