Dr. Shivani. B, Dr. Ila Srinivasan, Dr. Jyothsna. V. Setty, Dr. Neeraja . R


Aims and objectives: The present study was conducted to evaluate and compare antimicrobial efficacy of Xylitol and Stevia against S.mutans.
Methods: The bacterial suspensions of S.mutans were distributed over the Brain Heart Infusion agar using a sterile swab. 8 agar plates were
prepared. In each agar plate four wells of 6mm diameter were made of which two wells were utilized for xylitol and remaining two wells were
utilized for stevia. 100 microliters of xylitol and stevia solutions were introduced in to the wells. Plates were incubated at 37°C with 5% CO2 for
48hrs. After 48 hrs, zones of inhibition were measured, data were tabulated and subjected to statistical analysis. Student t-test was used.
Results: Both xylitol and stevia significantly inhibited the growth of S.mutans. xylitol exhibited significantly higher growth inhibition than stevia.
Conclusion: Xylitol exhibited a superior antibacterial activity compared to stevia. Unlike xylitol, stevia had modest, but significant inhibitory
effect on the growth of cariogenic S.mutans.


Stevia, Xylitol, S.mutans, Antimicrobial, Oral Health.

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Trahan L.Xylitol: a review of its action on Mutans streptococci and dental plaque--its clinical significance. Int Dent J1995; 45:77-92.

Michael W, Roberts J, Wright T. Non-nutritive, Low Caloric Substitutes for Food Sugars: Clinical Implications for Addressing the Incidence of Dental Caries and Overweight/Obesity. International Journal of Dentistry 2012:1-8.

Autio.JT.Effect of xylitol chewing gum on salivary Streptococcus mutans in preschool children.ASDC J Dent Child. 2002; 69:81-6.

Loesche WJ, Grossman NS, Earnest R, Corpron R.The effect of chewing xylitol gum on the plaque and saliva levels of Streptococcus mutans.J Am Dent Assoc. 1984; 108:587-92.

MatsukuboT, Takazoe I. Sucrose substitutes and their role in caries prevention. International Dental Journal 2012; 56: 119–130.

Debnath M. Clonal propagation and antimicrobial activity of an endemic medicinal plant Stevia rebaudiana. J. Med. Plants Res2008; 2:45-51.

Chatsudthipong V, Muanprasat C. Stevioside and related compounds: Therapeutic benefits beyond sweetness. PharmacolTher 2009; 121: 41-54.

MohammadiSichani, Maryam &Karbasizade, Vajihe&Aghai, Fatemeh&Mofid, Mohammad. Effect of different extracts of Stevia rebaudiana leaves on Streptococcus mutans growth. Journal of medicinal plant research 2012; 6:4731-4734.

Thomas JE, Glade MJ.Stevia: It’s Not Just About Calories.Open obesity journal 2010; 2:101-109.

Scheinin A, Kauko K. Mäkinen.Ylitalo K.Turku sugar studies I- An intermediate report on the effect of sucrose, fructose and xylitol diets on the caries incidence in man,ActaOdontologicaScandinavica 1974;32:383-412.

Söderling EM. Xylitol, mutans streptococci, and dental plaque. Adv Dent Res 2009; 21:74-78.

Bahador A, Lesan S, Kashi N. Effect of xylitol on cariogenic and beneficial oral streptococci: arandomized, double-blind crossover trial.Iran J Microbiol. 2012; 4: 75–81.

Kauko K, Mäkinen. Gastrointestinal Disturbances Associated with the Consumption of Sugar Alcohols with Special Consideration of Xylitol: Scientific Review and Instructions for Dentists and other health-care professionals. International Journal of Dentistry 2016: 1-15.

Saad A, Khan FA, Hayee A, Nazir MS. A Review on Potential Toxicity of Artificial Sweetnersvs Safety ofStevia: A Natural Bio-Sweetner. Journal of Biology, Agriculture and Healthcare 2014; 4:137-147.


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