Dr. Praveen Kumar, Dr Brajendra Kumar


The manual Mycobacteria Growth Indicator Tube (MGIT) method was evaluated for performing direct and indirect drug susceptibility testing
(DST) of Mycobacterium tuberculosis for isoniazid and rifampin on 101 strongly smear-positive sputum specimens in RIMS RAIPUR CG. Using
the indirect method of proportion (MOP) as the “gold standard,” the accuracies of isoniazid and rifampin susceptibility testing by the direct MGIT
system were 97.0 and 94.1%, respectively. The accuracy of the indirect MGIT system was 98.0% for both drugs. The turnaround times from
specimen processing to reporting of the DST results ranged between 4 and 23 (mean, 9.2) days by the direct MGIT method, 9 and 30 (mean, 15.3)
days by the indirect MGIT method, and 26 and 101 (mean, 59.6) days by the indirect MOP. MGIT appears to be a reliable, rapid, and convenient
method for performing direct and indirect DSTs in low-resource settings.


Liquid culture, Mycobacterium growth indicator tube, Tuberculosis

Full Text:



Bergmann, J. S., and G. L. Woods. 1997. Mycobacterial growth indicator tube for susceptibility testing of Mycobacterium tuberculosis to isoniazid and rifampin. Diagn. Microbiol. Infect. Dis. 28:153–156.

Bergmann, J. S., G. Fish, and G. L. Woods. 2000. Evaluation of the BBL MGIT (mycobacterial growth indicator tube) AST SIRE system for antimycobacterial susceptibility testing of Mycobacterium tuberculosis to 4 primary antituberculous drugs. Arch. Pathol. Lab. Med. 124:82–86.

Canetti, G., W. Fox, A. Khomenko, H. T. Mahler, M. K. Menon, D. A. Mitchison, N. Rist, and N. A. Sˇmelov. 1969. Advances in techniques of testing mycobacterial drug sensitivity, and the use of sensitivity tests in tuberculosis control programmes. Bull. W. H. O. 41:21–43.

Caviedes, L., T.-S. Lee, R. H. Gilman, P. Sheen, E. Spellman, E. H. Lee, D. E. Berg, S. Montenegro-James, and the Tuberculosis Working Group in Peru. 2000. Rapid, efficient detection and drug susceptibility testing of Mycobacterium tuberculosis in sputum by microscopic observation of broth cultures. J. Clin. Microbiol. 38:1203–1208.

Franzblau, S. G., R. S. Witzig, J. C. McLaughlin, P. Torres, G. Madico, A. Hernandez, M. T. Degnan, M. B. Cook, V. K. Quenzer, R. M. Ferguson, and R. H. Gilman. 1998. Rapid, low-technology MIC determination with clinical Mycobacterium tuberculosis isolates by using the microplateAlamar Blue assay. J. Clin. Microbiol. 36:362–366.

Frieden, T. R., L. F. Sherman, K. L. Maw, P. I. Fujiwara, J. T. Crawford, B. Nivin, V. Sharp, D. Hewlett, Jr., K. Brudney, D. Alland, and B. N. Kreisworth. 1996. A multi-institutional outbreak of highly drug-resistant tuberculosis: epidemiology and clinical outcomes. JAMA 276:1229–1235.

Heifets, L. 2000. Conventional methods for antimicrobial susceptibility testing of Mycobacterium tuberculosis, p. 133–143. In I. Bastian and F. Portaels (ed.), Multidrug-resistant tuberculosis. Kluwer Academic Publications, Amsterdam, The Netherlands.

Heifets, L., T. Linder, T. Sanchez, D. Spencer, and J. Brennan. 2000. Two liquid medium systems, Mycobacteria Growth Indicator Tube and MB Redox tube, for Mycobacterium tuberculosis isolation from sputum specimens. J. Clin. Microbiol. 38:1227–1230.

Huebner, R. E., R. C. Good, and J. I. Tokars. 1993. Current practices in mycobacteriology: results of a survey of state public health laboratories. J. Clin. Microbiol. 31:771–775.

Kent, P. T., and G. P. Kubica (ed.). 1985. Public health mycobacteriology: a guide for the level III laboratory. U.S. Department of Health and Human Services, Atlanta, Ga.


  • There are currently no refbacks.