Javad Chehri


Chitosan and its derivative water soluble Chitosan oligosaccharide are used in a variety of applications in pharmaceutical preparations. In this
study, 2 wild (ATCC 15729 and PAO1) and 2 mutant strains (PT121 and PT149) of P. aeruginosa are investigated for drug-drug interactions in
vitro. 10 antimicrobial agents (antibiotics) are combined with different degree of deacetylated Chitosans and Chitosan oligosaccharide. All the
chitosans show synergistic activity with sulfamethoxazole, a sulfonamide antimicrobial agent. It is interesting to observe that the MIC value for
the MexEF -OprN overexpressing mutant strain of P. aeruginosais 5 fold higher than the other strains under investigation suggesting a possible role
of this efux pump in Sulfamethoxazole efux. The ndings suggest on the use of chitosans as enhancing agent in combination with antibiotics in
pharmaceutical preparations.


Synergy, additive, combination therapy, Chitosan, chitin, drug resistance

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