HISTOPATHOLOGICAL CHANGES IN DEVELOPING MICE KIDNEY TREATED WITH LAMOTRIGINE ON EARLY AND LATE PHASE OF GESTATION

Shweta Singh, S.K. Pandey

Abstract


INTRODUCTION-Lamotrigine (LTG) is an anti-epileptic drug (AED) and also used as a neuromodulator, in mood
disorders.Metabolism is primarily achieved by competitive glucuronic acid conjugation. The principal product is an inactive
lamotrigine-2-N-glucuronide conjugate, found predominantly in urine and to a lesser extent in feces. Recently, malformations
have been reported in human foetuses, whose mothers were treated with LTG. However, it is not been possible to establish a
recognizable pattern of malformations in the kidneys of human foetuses treated with LTG, therefore the lower animal has been
used as experimental model for the present study.
OBJECTIVE- The objective of this study is to find out the microscopic changes in the kidney of the growing embryo of mice treated
with LTG in both early and late phase of gestation.
MATERIAL AND METHODS The pregnant mice were divided into two experimental groups i.e. early and late, each with two
subgroups i.e. control group (treated with intra peritoneal injection of normal saline on Day 4 and Day 9 of gestation) and treated
th th group (treated with intraperitoneal injection of LTG ,150mg/kg body weight on 4 day and 9 Day of gestation. Fetuses were
th collected on day 18 of gestation and after fixation the kidneys were dissected out and processed for histological examination.
RESULT - The results of this study indicate that LTG administered intra peritoneally at high doses causes shrinkage of cortex and
medulla and degenerated glomeruli, shrinkage of glomeruli and appearance of edematous spaces between the ducts.
CONCLUSION- In the present study histopathological changes were seen to be caused by LTG in the kidney of developing mice.


Keywords


Teratogenic, Lamotrigine, Microscopic, Kidney, Developing Mice.

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References


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